GMI: Arabidopsis immune system is triggered by small bacterial fatty acids

Like humans and animals, plants defend themselves against pathogens with the help of their immune system. But how do they activate their cellular defenses? Youssef Belkhadir's lab at the GMI studies cell-surface receptor kinases, which plants use to recognize external chemical signals. However, most of the chemicals these receptors react to are unknown. Together with an international group of researchers led by Stefanie Ranf at the Technical University of Munich, Youssef's lab has helped identify a new class of bacterial molecules that trigger the plant immune system.

Until now, scientists have thought that plant cells - similar to those of humans and animals - recognize bacteria through complex molecular compounds found in the bacterial cell wall. In particular, certain molecules composed of fat and sugar, lipopolysaccharides or LPS for short, were suspected of triggering the immune response. Ranf's team had previously identified the receptor kinase that recognizes LPS: lipo-oligosaccharide-specific reduced elicitation, or LORE for short.

However, as they set out to purify LPS to study the LPS:LORE interaction in more detail, they found that another class of small molecules, 3-hydroxy fatty acids, co-purified with the LPS. After finally seperating LPS from these fatty acids, they were surprised that the LPS did not trigger an immune response. Elwira Smakowska-Luzan, a postdoc in Youssef's lab, was able to show that LORE instead bound to the 3-hydroxy fatty acids. Further research confirmed that the 3-hydroxy fatty acids were both necessary and sufficient for triggering a potent immune response.

The 3-hydroxy fatty acids are very simple chemical building blocks compared to the much larger LPS. They are indispensable for bacteria and produced in large quantities for incorporation into diverse cellular components, thus making them attractive markers for recognition by the plant immune system.  In the future, these results could help in breeding or genetically engineering plants with an improved immune response. It is also possible that plants treated with 3-hydroxy fatty acids would have increased resistance to pathogens.


Bacterial medium chain 3-hydroxy fatty acid metabolites trigger immunity in Arabidopsis plants
Alexander Kutschera, Corinna Dawid, Nicolas Gisch, Christian Schmid, Lars Raasch, Tim Gerster, Milena Schäffer, Elwira Smakowska-Luzan, Youssef Belkhadir, A. Corina Vlot, Courtney E. Chandler, Romain Schellenberger, Dominik Schwudke, Robert K. Ernst, Stéphan Dorey, Ralph Hückelhoven, Thomas Hofmann, Stefanie Ranf
Science, April 12, 2019 – DOI: 10.1126/science.aau1279

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