SLAMseq vs. Standard RNA-Seq
SLAMseq can differentiate between newly synthesized (nascent) RNA and existing RNA, while standard RNA-Seq measures total steady-state RNA levels only.
Compared to standard RNA-Seq the SLAMseq protocol adds only two extra steps: labeling of the RNA by adding one compound to the culture medium and pre-processing the total RNA before continuing with a standard RNA-Seq protocol.
- Analyze transcriptome-wide kinetics of RNA synthesis and turnover
- Measure nascent RNA expression and transcript stability
- Gain novel insights into the control of gene expression
- Use in combination with QuantSeq 3’ mRNA-Seq for cost-effective, high throughput metabolic sequencing