The complexity of the human brain is largely due to its development involving processes unique to humans, many of which are still lurking in the darkest corners of our current scientific knowledge. Tuberous Sclerosis Complex (TSC) is no exception in this respect, as it has long been described as a chiefly genetic disorder based on data obtained from animal models. Now, breakthrough research from the Knoblich lab at IMBA – Institute of Molecular Biotechnology of the Austrian Academy of Sciences – uses patient-derived cerebral organoid models to pierce the mysteries of this rare neurodevelopmental disease. “Our findings on the root cause of TSC led us to a progenitor cell type specific to the human brain. This explains why the pathology of this disease could not be well established with other laboratory models,” explains IMBA Scientific Director Jürgen Knoblich, co-corresponding author on the publication.
In many affected patients, TSC manifests in the form of severe epilepsy and psychiatric symptoms like autism and learning difficulties. Morphologically, TSC is characterized by well-described signs often found in the brains of patients. Among those are benign tumors present in a defined area of the brain, as well as lesions in the brain cortex, or “cerebral mantle”, called “tubers”. For a long time, both morphological aberrations have been attributed to a genetic cause. However, results from the analysis of patient samples diverged from the prevalent theory, mainly with regards to tubers. “To study Tuberous Sclerosis, we developed cerebral organoid models of the disease: three-dimensional cell cultures that we use to model the brain and that we can derive from any patient,” explains co-corresponding author Nina Corsini, Research Associate in the Knoblich Group at IMBA.
Origin and involvement of CLIP cells in Tuberous Sclerosis pathology. Left: During midgestation, CLIP cells residing in the Caudal ganglionic eminence (CGE) generate interneurons that migrate into the cortex. Right: In Tuberous Sclerosis Complex (TSC), CLIP cells generate brain tumors and cortical tubers. Heterozygous mutations in TSC2 result in excessive proliferation of CLIP cells, generating cell types of cortical tubers (blue) as well as brain tumors (purple). ©Knoblich/IMBA.
Tumor on a Tuberous Sclerosis (TSC) patient-derived brain organoid. Immunostaining for mTOR activation marker pS6 (green) and the proliferation marker Ki67 (white in the nucleus). Organoid from TSC patient shows enlarged nodular tumor that extends out on the organoid surface at 110 days of age. ©Knoblich/IMBA.
For the study led by Corsini and Knoblich, the team grew brain organoids from several affected patients, a method that allows to investigate molecular and cellular mechanisms that existed in the patients’ brains at some point during development. “With this approach, we found that, like in the patients’ brains, the organoids grew tumors and had disorganized areas that resembled patient tubers,” explains Oliver Eichmüller, the first author on the study. However, recapitulating the pathophysiology of a disease is only the first step towards designating the culprit: “By digging further into the causes, we found that both of these abnormalities were triggered by the excessive proliferation of a cell type specific to the human brain,” states Eichmüller. These cells were termed Caudal Late Interneuron Progenitors, or CLIP cells. They are cells found during the developmental stage of human brains but not in animals like mice. “Our study shows that our brain is very complex – much more complex than the brains of most animals.”, says Corsini.
The scientists draw parallels to other neurodevelopmental and neuropsychiatric diseases, but also to malignant diseases affecting human brains, speculating that these could also be caused by human-specific developmental processes. “Our findings on human-specific principles in brain development and pathology could also apply to other known diseases for which no therapies exist to this date,” states Knoblich.
Having made headlines worldwide in 2013 for establishing human brain organoids at IMBA, the Knoblich lab already adapted this revolutionary technology to studying hidden processes of human brain development, as well as several diseases affecting the human brain. With their current findings, the team is now able to shed light on one of the shady slopes of neuroscience and medicine. “We will clearly not stop here!”, exclaims Knoblich. “As a next step, we aim to investigate further neuropsychiatric diseases by adapting our technology further. We are confident that this human-derived laboratory model will finally help us identify human-specific mechanisms that have been overlooked for far too long!”
Aggregates of dysmorphic interneurons in TSC patient-derived brain organoids. Immunostaining for human interneuron marker (Secretagogin) identifies aggregates of dysmorphic interneurons in human cerebral organoids derived from TSC patients that are around 230 days old. Note that dysmorphic neurons show enlarged cell body and processes. ©Knoblich/IMBA.
Eichmüller O.L., Corsini N.S., et al., “Amplification of human interneuron progenitors promotes brain tumors and neurological defects”, Science, 2022. DOI: https://doi.org/10.1126/science.abf5546
IMBA - Institute of Molecular Biotechnology - is one of the leading biomedical research institutes in Europe focusing on cutting-edge stem cell technologies, functional genomics, and RNA biology. IMBA is located at the Vienna BioCenter, the vibrant cluster of universities, research institutes and biotech companies in Austria. IMBA is a subsidiary of the Austrian Academy of Sciences, the leading national sponsor of non-university academic research. The stem cell and organoid research at IMBA is being funded by the Austrian Federal Ministry of Science and the City of Vienna.