This translational project combines cutting edge molecular research at the level of kinome, gene expression, and microRNome in samples of patients who suffered from sepsis and of animal models of this disease. In both human and mouse lungs and kidneys we will enrich the samples for microvasculature using laser microdissection (LMD) and next analyze the status of the cellular signal transduction status (kinome) and microRNA levels in an unbiased -omics approach, with dedicated mRNA levels as reference.
Our new approach will identify, evaluate, and validate molecular targets in, and biomarkers of failing kidneys and lungs in sepsis and provide an microvasculature, organ specific molecular signature of sepsis related organ dysfunction. This project will deliver a blueprint for a novel approach in drug development for use in other complex diseases in which zooming in on cell types in tissues that matter enables the unmasking of the molecular basis of disease.
UMCG is a 1339 bed Academic Medical Center in the Netherlands, providing top level medical care to approx. 3 million inhabitants of the Northern & Eastern Netherlands and Northern Germany. On top of that UMCG provides education to 3,500 medical students & delivers state of the art R&D to the medical community via 1,000 researchers. In research the UMCG is focusing on ‘healthy ageing´.
TAmiRNA specializes in technologies for profiling levels of blood-circulating microRNAs and developing multi-parametric classification algorithms (“signatures”). TAmiRNA uses these technologies to develop minimal-invasive diagnostic tests for drug development, early diagnosis and prognosis of disease, and as companion diagnostic tests to support treatment decisions.
Vivomicx has developed validated protocols to analyze preclinical and clinical tissue samples using Laser Dissection Microscopy in combination with quantitative RT-PCR analysis for in-depth analysis of gene expression in subsets of cells. Using this technology we unmask the molecular identity of cells in the tissue context. In this way we determine e.g., whether target molecules are expressed in cells of interest in diseased tissue and which responding molecules can serve as genuine biomarkers of disease activity/drug effects.
The financial support for this R&D PPP-Allowance project was granted through the Topconsortia for Knowledge & Innovation’s (TKI) office of the Topsector Life Sciences & Health (Health-Holland)