Topics will include
- Reprogramming of human dermal fibroblasts to iPSCs
- Feeding, passaging and assessment of murine and human ESC cultures
- Freezing and thawing of ES cells
- Gene editing of mouse and human embryonic stem cells using CRISPR/Cas9 technology
This course is highly practical and will be based in a Class II tissue culture facility. All relevant safety equipment, clothing and training will be provided.